Determining lysis in a solution

Experiment 1: Effect of distilled water and isotonic saline

Scientific study involves the collection of quantitative data and also often the recording of qualitative observations. These can be as informative as quantitative measurements, and often serve to provide insights that help guide future experiments. Here we require you to collect this type of observational data.


  1. Add one of the solutions (either distilled water or isotonic saline) to the test tube.
  2. Now add some blood to the test tube.
  3. Observe whether lysis occurs to the RBCs in the solution, inputting your findings in the table.
  4. Reset the experiment to test the other solution.
Solution Did lysis occur?
Distilled water
Isotonic saline