Slowing down lysis
Experiment 2: Effect of hypotonic saline (50% saline)
Because biological systems are stochastic not deterministic, there will be variation from trial to trial in the responses elicited from a system, for a variety of reasons. Hence, it is important to repeat the same test a number of times to obtain the mean response of the system and determine reproducibility.
NOTE: This model assumes that the system is invariant from trial to trial and this is, as is clear for many biological systems, often not a good assumption to make.
- Add 2.5mL of each solution into the test tube to make 5mL of 50% Physiological saline.
- Now add a couple of drops of blood into the test tube.
- A timer will start automatically. Stop the timer when the solution inside the tube becomes clear enough to see the lightbulb filament.
- Record how long it takes for lysis to occur in the table below. Repeat the experiment two more times to calculate the average.
|Trial 1||Trial 2||Trial 3||Mean|